《植物生理学报》 2012, 48(3): 247-250

香石竹斑驳病毒(CarMV)脱除对几种生理指标的影响

王丽花¹, 吴学尉¹, 杨秀梅², 瞿素萍², 王继华^1,*, 莫锡君¹

云南省农业科学院1花卉研究所; 2农业部花卉产品质量监督检验测试中心(昆明), 昆明650205

通信作者：王继华；E-mail: wjh0505@gmail.com；Tel: 0871-5895788

摘　要：

感染病毒的香石竹组培苗于(38.5±1) ℃条件下热处理29 d后, 切取0.1~0.2 mm的茎尖生长点无菌培养成苗后, 经DASElisa检测获得脱除香石竹斑驳病毒(Carnation mottle virus, CarMV)的香石竹脱毒苗, 取脱毒苗与非脱毒苗的叶片, 测量叶绿素a、叶绿素b、类胡萝卜素、丙二醛(MDA)、干物质含量几种生理生化指标。结果表明, 脱毒苗与非脱毒苗相比: (1)叶绿素和类胡萝卜素含量增加, 具有较强的光合效率; (2)与抗性生理有关的MDA含量下降, 衰老减缓; (3)光合物质的形成与积累增多, 干物质含量增加

关键词：香石竹斑驳病毒; 脱毒; 生理指标

收稿：2011-10-25   修定：2012-02-09

资助：国家“863”计划课题(2011AA100208)和云南省重点新产品开发计划(2011BB013)。

Production of CarMV-Free Carnation Plants and Its Effects on Some Physiological Indicators

WANG Li-Hua¹, WU Xue-Wei¹, YANG Xiu-Mei², QU Su-Ping², WANG Ji-Hua^1,*, MO Xi-Jun¹

¹Flower Research Institute; ²Supervision and Testing Center for Flowers and Ornamental Plant Quality, Ministry of Agriculture, Yunnan Academy of Agricultural Sciences, Kunming 650205, China

Corresponding author: WANG Ji-Hua; E-mail: wjh0505@gmail.com; Tel: 0871-5895788

Abstract:

The common tissue culture seedlings were cultured in heat-treatment conditions (38.5±1) ºC for 29 d, then the stem-apexes were cut at 0.1-0.2 mm and cultured in sterile condition. Carnation mottle virus (CarMV)-free carnation plants were obtained which had been detected CarMV by DAS-Elisa. Leaves of CarMV-free seedlings and common seedlings were cut down and measured the contents of chlorophyll a, chlorophyll b, carotenoids, malondialdehyde (MDA) and dry-matter. The results showed that comparative study between CarMV-free seedlings and common seedlings: (1) chlorophyll and carotenoids content of CarMV-free seedlings increased, and the photosynthetic efficiency was high; (2) MDA content which relate to the physiological resistance of CarMV-free seedlings decreased, delaying aging; (3) formation and accumulation of photosynthates increased that could cause more dry-matter content.

Key words: Carnation mottle virus (CarMV); virus-elimination; physiological indicators